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CAZyme Information: MGYG000000605_00409

You are here: Home > Sequence: MGYG000000605_00409

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species
Lineage Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Ruminococcus_C;
CAZyme ID MGYG000000605_00409
CAZy Family GH5
CAZyme Description hypothetical protein
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
604 67536.04 4.1056
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000000605 2224694 MAG Madagascar Africa
Gene Location Start: 17270;  End: 19084  Strand: -

Full Sequence      Download help

Enzyme Prediction      help

EC 3.2.1.4

CAZyme Signature Domains help

Family Start End Evalue family coverage
GH5 70 350 5.2e-89 0.9891304347826086

CDD Domains      download full data without filtering help

Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
pfam00150 Cellulase 3.85e-63 55 351 1 270
Cellulase (glycosyl hydrolase family 5).
COG2730 BglC 9.53e-32 39 366 45 376
Aryl-phospho-beta-D-glucosidase BglC, GH1 family [Carbohydrate transport and metabolism].
pfam18259 CBM65_1 8.74e-11 420 518 14 113
Carbohydrate binding module 65 domain 1. This domain is found in the non-catalytic carbohydrate binding module 65B (CMB65B) present in Eubacterium cellulosolvens. CBMs are present in plant cell wall degrading enzymes and are responsible for targeting, which enhances catalysis. CBM65s display higher affinity for oligosaccharides, such as cellohexaose, and particularly polysaccharides than cellotetraose, which fully occupies the core component of the substrate binding cleft. The concave surface presented by beta-sheet 2 comprises the beta-glucan binding site in CBM65s. C6 of all the backbone glucose moieties makes extensive hydrophobic interactions with the surface tryptophans of CBM65s. Three out of the four surface Trp are highly conserved. The conserved metal ion site typical of CBMs is absent in this CBM65 family.
cd14256 Dockerin_I 0.003 547 594 1 50
Type I dockerin repeat domain. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. The cellulosome consists of scaffoldin, a noncatalytic scaffolding polypeptide, that comprises repeating cohesion modules and a single carbohydrate-binding module (CBM). Specific calcium-dependent interactions between cohesins and dockerins appear to be essential for cellulosome assembly. This subfamily represents type I dockerins, which are responsible for anchoring a variety of enzymatic domains to the complex.

CAZyme Hits      help

Hit ID E-Value Query Start Query End Hit Start Hit End
ADU86907.1 3.53e-144 36 506 37 488
QPB75694.1 1.13e-141 2 580 3 563
CAB05881.1 2.49e-127 7 531 9 549
AAB19708.1 2.12e-116 30 422 30 436
ADU21113.1 9.10e-98 4 392 6 382

PDB Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
6XSU_A 1.05e-125 29 387 4 353
GH5-4broad specificity endoglucanase from Ruminococcus flavefaciens [Ruminococcus flavefaciens],6XSU_B GH5-4 broad specificity endoglucanase from Ruminococcus flavefaciens [Ruminococcus flavefaciens]
6Q1I_A 2.22e-78 27 373 3 341
GH5-4broad specificity endoglucanase from Clostrdium longisporum [Clostridium longisporum],6Q1I_B GH5-4 broad specificity endoglucanase from Clostrdium longisporum [Clostridium longisporum]
6WQP_A 4.07e-73 36 372 14 343
GH5-4broad specificity endoglucanase from Ruminococcus champanellensis [Ruminococcus champanellensis],6WQP_B GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis [Ruminococcus champanellensis],6WQV_A GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose [Ruminococcus champanellensis],6WQV_B GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose [Ruminococcus champanellensis],6WQV_C GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose [Ruminococcus champanellensis],6WQV_D GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose [Ruminococcus champanellensis]
3AYR_A 5.51e-73 36 371 18 346
GH5endoglucanase EglA from a ruminal fungus [Piromyces rhizinflatus]
3AYS_A 4.18e-72 36 371 18 346
GH5endoglucanase from a ruminal fungus in complex with cellotriose [Piromyces rhizinflatus]

Swiss-Prot Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
P54937 1.35e-76 27 373 28 366
Endoglucanase A OS=Clostridium longisporum OX=1523 GN=celA PE=1 SV=1
P23660 1.08e-72 36 384 25 360
Endoglucanase A OS=Ruminococcus albus OX=1264 GN=celA PE=1 SV=1
P23661 1.20e-69 31 373 64 388
Endoglucanase B OS=Ruminococcus albus OX=1264 GN=celB PE=3 SV=1
P16216 8.31e-69 31 373 62 386
Endoglucanase 1 OS=Ruminococcus albus OX=1264 GN=Eg I PE=1 SV=1
P20847 7.56e-67 37 372 38 382
Endoglucanase 1 OS=Butyrivibrio fibrisolvens OX=831 GN=end1 PE=3 SV=1

SignalP and Lipop Annotations help

This protein is predicted as SP

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
0.000328 0.998858 0.000224 0.000199 0.000192 0.000167

TMHMM  Annotations      download full data without filtering help

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