peptidase C58 domain of Photorhabdus asymbiotica toxin PaTox and LifA/Efa1-related large cytotoxin, and similar proteins. This subfamily includes the cysteine protease domain of Photorhabdus asymbiotica toxin PaTox, a large virulence-associated multifunctional protein toxin. This domain is similar to AvrPphB protease found in Pseudomonas syringae, a C58 protease. Mutation studies show that this domain enhances cytotoxic effects of the toxin, and therefore is essential for full PaTox activity. Also included in this family is the enteropathogenic Escherichia coli (EPEC) factor for adherence/lymphocyte activation inhibitor (efa1/lifA) gene which is strongly associated with diarrhea. Efa1/LifA proteins are important for A/E lesion formation efficiency in EPEC strains lacking multiple effectors. This domain contains the invariant C/H/D residues conserved in the C58/YopT family.

LifA/Efa1-related large cytotoxin. Members of this family are large and almost certainly multifunctional proteins found in various pathogens from genus Chlamydia, about 3000 amino acids in size and related to lymphostatin (Efa1/LifA) from enteropathogenic Escherichia coli. Roles have been suggested for Efa1 (EHEC factor for adherence) in adhesion, so some members have been annotated as adherence proteins rather than cytotoxins.

peptidase C58-like domain of cytotoxin RtxA and similar proteins. This subfamily includes the C58 peptidase-like domain of Vibrio vulnificus biotype 3 multifunctional autoprocessing RTX (MARTX) toxin, the primary virulence factor of V. vulnificus. MARTX has been shown to be an essential virulence factor contributing to highly inflammatory skin wounds with severe damage affecting every tissue layer. This toxin is a large single-polypeptide composed of repeat sequences that form a pore in eukaryotic cell plasma membranes for the translocation of centrally located effector domains. This C58 family cysteine protease domain usually contains the invariant C/H/D residues that form an active site triad, however, cysteine is not fully conserved in this group.

peptidase C58 domain. The C58 family peptidases are endopeptidases that also act as transamidases, attaching a lipid moiety to the newly exposed N-terminus of the substrate. These include the Pseudomonas avirulence (Avr) protein AvrPphB and the homologous protein from Yersinia known as YopT; both are involved in bacterial pathogenesis. These proteins have a papain-like fold and a distinct substrate-binding site. The proteolytic activity of AvrPphB is essential for autoproteolytic cleavage of an AvrPphB precursor as well as for eliciting the hypersensitive response in plants. Yersinia pestis YopT cleaves the post-translationally modified Rho GTPases near their carboxyl termini, releasing them from the membrane. This leads to the disruption of actin cytoskeleton in host cells. Also included in this family is the Pseudomonas syringae HopN1 peptidase, a type III secretion system effector that can suppress plant cell death events in both compatible and incompatible interactions. All of these proteolytic activities are dependent upon the invariant C/H/D residues conserved in the C58/YopT family peptidase domain.

peptidase C58 domain of the YopT subfamily, including Yersinia pestis YopT and related proteins. This subfamily includes the plague organism Yersinia pestis cysteine protease YopT, an outer membrane protein. Y. pestis can disarm the host immune response by interfering with cell-signaling pathways; YopT cleaves post-translationally modified Rho GTPases near their carboxyl termini, releasing them from the membrane. This leads to the disruption of the actin cytoskeleton in host cells. YopT's proteolytic activity is dependent upon the invariant C/H/D residues conserved in the C58/YopT family peptidase domain.

Crystalstructure of alpha-beta hydrolase (ABH) and Makes Caterpillars Floppy (MCF)-Like effectors of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II2_B Crystal structure of alpha-beta hydrolase (ABH) and Makes Caterpillars Floppy (MCF)-Like effectors of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II2_C Crystal structure of alpha-beta hydrolase (ABH) and Makes Caterpillars Floppy (MCF)-Like effectors of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II2_D Crystal structure of alpha-beta hydrolase (ABH) and Makes Caterpillars Floppy (MCF)-Like effectors of Vibrio vulnificus MO6-24/O [Vibrio vulnificus]

Crystalstructure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II0_B Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II0_C Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II0_D Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II6_A Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O in complex with a human ADP-ribosylation factor 3 (ARF3) [Vibrio vulnificus],6II6_B Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O in complex with a human ADP-ribosylation factor 3 (ARF3) [Vibrio vulnificus]

Multifunctional-autoprocessing repeats-in-toxin OS=Vibrio vulnificus OX=672 GN=CRN52_02910 PE=1 SV=1